Fig. 5
From: Curcumin mitigates high glucose-induced cardiac oxidative stress via Notch1 pathway activation
CUR activated Notch1 signaling pathways. (A, B) Rat myocardial cells were incubated in HG-treated with or without CUR for 36 h, respectively. qRT-PCR was applied to detect the mRNA expression of Notch1 and Hes1 genes. (C) Notch1, N1ICD and Hes1 protein expression were evaluated by western blotting; β-actin was used as an internal control. (D) DAPT and CUR affected the translocation of N1ICD from cytoplam to the nucles.CUR promoted N1ICD translocation into the nucleus. (E-G) The expression levels of Notch1, N1ICD and Hes1 proteins significantly increased in the HG + CUR group (**P < 0.01 vs. HG group) and decreased in both the HG + CUR + DAPT (**P < 0.01 vs. HG + CUR group). (H) The expression levels of N1ICD proteins in nuclear significantly increased in both the HG + CUR group (**P < 0.01 vs. HG group, and **P < 0.01 vs. HG + CUR + DAPT). Data are expressed as the mean ± SD, n = 3.
