Table 5 Functional prediction of nitrogen metabolism and arginine biosynthesis in soil microbiomes from paddy soils in Kaya and Ishikawa villages.

From: Soil microbes and organic fertilizer efficiency are associated with rice field topography

Function

Description

METACYC

KEGG ortholog

Relative frequency (%)

Probability

Kaya

Ishikawa

Nitrogen metabolism

Nitrate reduction I (denitrification)

DENITRIFICATION-PWY

–

0.0173

0.0125

0.016

*

 

Nitrate reductase / nitrite oxidoreductase, alpha subunit [1.7.5.1 1.7.99.-]

–

K00370

0.0066

0.0043

0.0004

***

 

Nitrate reductase / nitrite oxidoreductase, beta subunit [1.7.5.1 1.7.99.-]

–

K00371

0.0067

0.0044

0.0004

***

 

Nitrate reductase gamma subunit [1.7.5.1 1.7.99.-]

–

K00374

0.0065

0.0045

0.001

***

 

Nitrite reductase (NO-forming) [1.7.2.1]

–

K00368

0.0019

0.0013

0.04

*

 

Nitric oxide reductase subunit B [1.7.2.5]

–

K04561

0.0068

0.0056

0.021

*

 

Nitrous-oxide reductase [1.7.2.4]

–

K00376

0.0015

0.0009

0.011

*

Arginine biosynthesis

Urea cycle

PWY-4984

–

0.2276

0.1570

0.002

**

 

Ornithine carbamoyltransferase [2.1.3.3]

–

K00611

0.0638

0.0625

0.272

 
 

Argininosuccinate synthase [6.3.4.5]

–

K01940

0.0692

0.0668

0.041

*

 

Argininosuccinate lyase [4.3.2.1]

–

K01755

0.0692

0.0668

0.042

*

 

Arginase [3.5.3.1]

–

K01476

0.0150

0.0090

0.005

**

  1. Functional compositions were analyzed by the METACYC and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The difference in relative frequency between Kaya and Ishikawa was tested by t-test. ‘***’, ‘**’, and ‘*’ indicate 0.1, 1, and 5% levels of significance, respectively.