Fig. 6
Spheroid formation assay in irradiated vs. non-irradiated A549 cells in the presence vs. absence of EPHA2 and CTSD inhibitors. (A, B) Representative examples of spheroids formed during 96 h post-seeding. Data from a single experiment is shown; the treatment conditions are listed in the top right corner of each panel. Scale bar (top left): 400 μm. (C, D) Pooled normalized area of spheroids at 96 h post-seeding (N = 2 for regorafenib and N = 5 for other compounds); the treatment conditions are listed below the graphs. In (A) and (C), NT stands for non-treated. Each column represents average ± standard error of mean. Statistical significance of the grouped comparisons (Kruskal-Wallis test): *** corresponds to P < 0.001; ** corresponds to P < 0.01; * corresponds to P < 0.05; ns, not significant; arrows indicate the treatments (non-treated A549 or irradiated A549) to which other treatments are compared. Statistical significance of paired comparisons (Mann-Whitney test without multiple comparison correction): (***) corresponds to P < 0.001; (**) corresponds to P < 0.01; * corresponds to P < 0.05; ns, not significant. Abbreviations: ALW, ALW-II-41-27; pepstatin, pepstatin A.
