Fig. 2
THBS2 promotes an aggressive phenotype in human lung fibroblasts. (A) N-FBs were transfected with THBS2 plasmid and the expression level of THBS2 protein was detected by Western blot. n = 3 for each group. Data are shown as the mean ± SEM. **P < 0.01. (B) Transwell chamber was used to detect the effect of THBS2 transfection on the migration ability of N-FBs cells. Scale bar, 200μm. n = 3 for each group. Data are shown as the mean ± SEM. ***P < 0.001. (C) EdU assay was used to detect the proliferation ability of N-FBs stimulated by THBS2 plasmid and negative control vector. Scale bar, 100 μm. n = 3 for each group. Data are shown as the mean ± SEM. **P < 0.01. (D) IPF-FBs with THBS2 siRNA (si-THBS2#2) was transfected and the expression level of THBS2 protein was detected by Western blot. n = 6 for each group. Data are shown as the mean ± SEM. **P < 0.01. (E) Forty-eight hours after transfection of THBS2 siRNA (si-THBS2#2) and NC siRNA (si-NC), equal numbers of fibroblasts were loaded into transwell chambers. Images of migrating fibroblasts from the crystal violet staining assay are shown. Scale bar, 200 μm. n = 6 for each group. Data are shown as the mean ± SEM. ****P < 0.0001. (F) Proliferation of THBS2-deficient cells. After transfection of THBS2 siRNA (si-THBS2#2) or NC siRNA (si-NC) for 48 h, EdU method was used to detect the proliferation of IPF-FBs cells. Scale bar, 100 μm. n = 6 for each group. Data are shown as the mean ± SEM. ****P < 0.0001.
