Table 2 Polymorphisms detected in porin OprD of the 13 carbapenem-resistant PA-BSI.

From: Impact of the COVID-19 pandemic on the epidemiology and molecular features of Pseudomonas aeruginosa bloodstream infections

Strain

Isolation period

OprD size (aa)a

Amino acid changesa

Insertion/deletion

Ps1016

Pre-COVID

93

–

Deletion of 1 nt (C) at codon 86 (nt 258)

Ps1019

Pre-COVID

–

–

ISPa104 at codon 378 (nt 1132)

Ps1020

Pre-COVID

441

D43N, S57E, S59R, E202Q, I210A, E230K, S240T, N262T, A267S, A281G, K296Q, Q301E, R310G, V359L

Loop L7-short

Ps1028

Pre-COVID

441

D43N, S57E, S59R, E202Q, I210A, E230K, S240T, N262T, A267S, A281G, K296Q, Q301E, R310G, V359L

Loop L7-short

Ps1031b

Pre-COVID

441

D43N, S57E, S59R, E202Q, I210A, E230K, S240T, N262T, A267S, A281G, K296Q, Q301E, R310G

Loop L7-short

Ps1040

Pre-COVID

343

D43N, S57E, S59R, E202Q, I210A, E230K, S240T, N262T, A267S, A281G

Deletion of 4 nts (CTAC) at codon 293 (nt 879)

Ps1043

Pre-COVID

276

D43N, S57E, S59R, E202Q, I210A, E230K, S240T, N262T, A267S, W277*

–

Ps1047

Pre-COVID

276

D43N, S57E, S59R, E202Q, I210A, E230K, S240T, N262T, A267S, W277*

–

Ps1056

Pre-COVID

63

D43N, S57E, S59R, W64*

–

Ps1317

Post-COVID

443

–

–

Ps1448

Post-COVID

 > 443

T103S, K115T, F170L, A247T

Deletion of 5 nts (CCGGT) at codon 402 (nts 1208–1212)

Ps1450

Post-COVID

276

G60R, T105A, V127L, E185Q, P186G, V189T, E202Q, I210A, E230K, S240T, S248E, N262T, A267S, W277*

–

Ps1459

Post-COVID

431

D43N, S57E, S59R, E202Q, I210A, E230K, S240T, A247T, N262T, A267S, A281G, K296Q, Q301E, R310G, V359L

Deletion of 1 nt (A) at codon 366 (nt 1099), loop L7-short

  1. aThe substitutions were determined by comparison with the sequence of P. aeruginosa PAO1 control strain (GenBank accession number AE004091) that encodes a protein of 443 amino acids (aa).
  2. bMBL-positive PA.
  3. Abbreviations: *Premature stop codon, nt: nucleotide, loop L7-short: Shortening of putative Loop L7: 372 V-DSSSSYAGL-L383.
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