Fig. 5 | Scientific Reports

Fig. 5

From: Guilu erxian glue improves sperm quality in oligasthenospermia mice by promoting TDRP methylation and regulating the cAMP/PKA pathway

Fig. 5

GLEXG enhances the vitality of H2O2-treated GC-2 cells by promoting the methylation of TDRP. A qRT-PCR analysis of TDRP levels. B Detection of cAMP and PKA expression. C Methylation of TDRP gene in testicular tissue examined by MSP. D Assessment of cell viability using the CCK-8 assay. E Measurement of cell migration using the Transwell assay (magnification: ×100, scale bar = 100 μm). F Flow cytometry analysis of mitochondrial membrane potential. G Biochemical measurement of intracellular ATP levels. *P < 0.05 vs. Control; #P < 0.05 vs. H2O2-treated; &P < 0.05 vs. H2O2-treated + GLEXG; n = 3. The data are presented as mean ± SD.

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