Table 1 Primer sequences.

From: Guilu erxian glue improves sperm quality in oligasthenospermia mice by promoting TDRP methylation and regulating the cAMP/PKA pathway

Gene

Sequence

Length

TDRP

F AAGTCCAAAGGAACTAACCAACG

76 bp

R CCAAGGCGTCCCAAAATC

TDRP (for MSP)

F AGTTTTTAGTGTTATTTTAAAGCGG

R AAACTACATCCTACCAAAATCGAA

131 bp

PKA

F TGAGCAAAGGCTACAACAAG

R GTCAGCGAAGAAGGGTGG

92 bp

β-actin

F ACCCTGAAGTACCCCATCGAG

224 bp

R AGCACAGCCTGGATAGCAAC

  1. F: forward; R: reverse; MSP: methylation-specific PCR.
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