Fig. 6
From: Machine learning identifies KRT8 dysregulation and endothelial remodeling in Moyamoya disease
Overexpression of KRT8 promotes tubule formation and enhances migratory capacity in HBMECs. (A) The tubule formation assay of HBMEC cells (Bar = 200 μm). (B) Validation of transfection efficiency by qPCR. The expression level of KRT8 in the oe-vector group was significantly higher than that in the control group. The expression level of KRT8 in the oe-KRT8 group was significantly higher than that in both the control group and the oe-vector group. White represents the control group. Blue represents the oe-vector group. Red represents the oe-KRT8 group. *p < 0.05, **p < 0.01. (C) Number of tubule branches. The number of tubule branches in the oe-KRT8 group was significantly higher than that in the oe-vector group. White represents the control group. Blue represents the oe-vector group. Red represents the oe-KRT8 group. *p < 0.05, **p < 0.01. (D) Total tubule length. The total tubule length in the oe-KRT8 group was significantly longer than that in the oe-vector group. White represents the control group. Blue represents the oe-vector group. Red represents the oe-KRT8 group. *p < 0.05, **p < 0.01. E: The number of migrating cells corresponding to different groups. The number of migrating cells in the oe-KRT8 group was significantly higher than that in the oe-vector group. *p < 0.05, **p < 0.01. F: Stained images of migrating cells in different groups under a microscope *p < 0.05, **p < 0.01. (Bar = 100 μm).
