Fig. 3
Overview of the coding capacity of plasmids used in the transient expression assays. (a) The coding sequence for the SARS-CoV-2 NSP1-NSP2 plus a C-terminal Flag-tag and a stop codon were inserted into the pGEM-3Z plasmid, containing the T7 promoter upstream of the insert. The lengths of both NSP1 and NSP2 (in terms of the number of amino acid residues) are indicated, and the location of the conserved motif (WVPRA) is marked. This plasmid is referred to as the wt. (b) The NSP3-mCherry construct, encoding a functional PLpro, was a gift from Bruno Antonny (see Materials and Methods)14, a T7 promoter sequence was inserted into this plasmid, upstream of the coding sequence for NSP3, as described in Materials & Methods. Created in BioRender. Kristensen, T. (2025) https://BioRender.com/d9lyyyv.
