Fig. 1

Suppression of mig-17 and mig-18 DTC migration defects by rlp-20(tk73). (A) Gonad morphology (arrows) of wild type, mig-17(k174), and mig-17(k174); rpl-20(tk73) young-adult hermaphrodites. Posterior gonads are shown. Anterior to the left, dorsal to the top. Bar, 20 μm. (B) Quantitative analysis of mig-17 gonadal defects combined with rpl-20(tk73). N = 60 for each strain. P-values for Fisher’s exact test against mig-17(k174) for mig-17(k174) carrying strains are indicated: ***, P < 0.001; NS, not significant. (C) Transgenic rescue experiments of mig-17. mig-17 animals introduced with transgenic arrays containing PCR-amplified fragments of the rpl-20(tk73) gene were suppressed for their gonadal defects. #1 and #2 are independently established transgenic lines. N = 60 for each strain. P-values for Fisher’s exact test against mig-17(k174) are indicated: ***, P < 0.001. (D) Amino acid sequence homology between RPL-20 and human RPL18a. Identical amino acids are shown in black boxes. G82 mutated in rpl-20(tk73) is depicted with an arrow. (E-G) rpl-20(tk73) suppresses the DTC migration defects of mig-17 and mig-18 alleles (E), but not gon-1(RNAi) (F) or sqv-5(k175) and mig-22(k141) mutants (G). sqv-5(k175) and mig-22(k141) were marked with unc-13(e1091) and unc-32(e189), respectively. N = 60 for each strain. P-values for Fisher’s exact test against mig-17, mig-18, rpl-20(tk73) control RNAi, sqv-5, and mig-22 for the respective double mutants are indicated: ***, P < 0.001; **, P < 0.01; NS, not significant.