Fig. 3

Tissue-specific expression of RPL-20. (A) Expression of mCherry-RPL-20(WT) and mCherry-RPL-20(tk73). Upper panels are Nomarski and confocal merged images for young adult animals and lower panels represent close-up images of the gonadal tip regions. Arrows and arrowheads indicate intestine and the distal tip cells, respectively. Bars, 50 μm. (B, C) Rescue experiments of the mig-17 gonadal defect by expressing rpl-20(tk73) (B) or rpl-20(WT) (C) genes under various tissue-specific promoters. N = 60 for each strain. P-values for Fisher’s exact test against mig-17(k174) for mig-17(k174) carrying strains are indicated: ***, P < 0.001; **, P < 0.01; NS, not significant. #1 and #2 are independently established transgenic lines. (D, E) Growth rate analysis of mig-17 animals carrying various plasmid constructs. Percentages of larval stages were determined at 43 h (D) and 48 h (E) after hatching, as described in Methods. #1 and #2 are independently established transgenic lines. Color codes represent larval stages, early L4 (EL4), mid-L4 (ML4), mid-late L4 (MLL4), late L4 (LL4), and adult (A), respectively. N ≥ 60 for each strain.