Fig. 4 | Scientific Reports

Fig. 4

From: Ribosomal protein mutation suppresses gonadal leader cell migration defects in mig-17/ADAMTS mutants in Caenorhabditis elegans

Fig. 4

Analysis of ribosomes. (A) Western blot analysis of ribosomes for wild type animals expressing mCherry-RPL-20(tk73) or mCherry-RPL-20(WT). Ribosomes were precipitated by dual sucrose cushion centrifugation and the samples of supernatants and precipitates were analyzed. Sup 1 and 2 and PPT 1 and 2 represent supernatant and precipitate samples from first and second centrifugation, respectively. Arrow indicates the bands corresponding to mCherry-RPL-20. The double bands may be generated due to post-translational modification of RPL-2044. Original blot is presented in Supplementary Figure S3. (B) Polysome profiles of wild type and rpl-20(tk73) animals. The amounts of 60 S subunit and 80 S ribosome in rpl-20(tk73) were reduced relative to the wild type.

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