Fig. 1
From: Constitutive interaction between neutrophils and von Willebrand factor in peripheral blood
Interaction between mouse peripheral neutrophils and VWF. (A) Representative flow cytometry histogram illustrating VWF presence on the surface of neutrophils from wildtype mice. (B) Statistical analysis of mean fluorescence intensity (MFI) for VWF on neutrophil surfaces relative to control (n = 11–12). (C) SDS-agarose gel electrophoresis comparing VWF multimer in neutrophils (Ly6G+) versus other leukocytes isolated from wildtype mouse peripheral blood. Full-length gel is included in supplementary figure S5A. (D) Flow cytometry scatter plot displaying leukocyte populations from GFP-VWF transgenic mice stained with anti-Ly6G antibody. (E) Overlay of flow cytometry histograms comparing VWF signal intensity between platelets and neutrophils in GFP-VWF transgenic mice. (F) Statistical summary (n = 4) corresponding to data in preceding panels. Statistical significance was assessed using t-tests for pairwise comparisons and one-way ANOVA for multiple groups, with significance denoted as ****p < 0.0001, *p < 0.05.
