Figure 2

Key 2D NMR data and literature comparisons leading to the structural revision of scytonemin imine. (a) Key HMBC and NOESY couplings that led to the reassignment of scytonemin imine as 2b, including 4/5-bond HMBC couplings that are inconsistent with the originally reported structure 2a and an isomeric cyclic imine. (b) The structure of alstrobrogaline (3) with 13C chemical shift comparisons to 2b. (c) the reassigned structure of scytonemin imine (2b) with full numbering. Created in BioRender. Jeffrey, C. (2025) https://BioRender.com/hdfwnqm.