Fig. 4
Determination of intracellular uric acid and triglyceride concentrations, and Western blotting analysis after cells and spheroids are exposed to fructose (0 mM or 20 mM) for 72 h. a Intracellular uric acid levels of Huh monolayer. b Intracellular triglyceride levels of Huh monolayer. c Intracellular uric acid levels of Huh spheroids. d Intracellular triglyceride levels of Huh spheroids. Values of uric acid and triglycerides are means ± SEM of three replicates. *p < 0.05, **p < 0.01, ***p < 0.005, and ****p < 0.0001. e Representative images of western blotting analysis of protein lysate of Huh monolayer. f Representative images of western blotting analysis of protein lysate of Huh spheroids. AMP kinase (AMPK), Acetyl-CoA carboxylase (ACC), phosphorylated protein (p).
