Fig. 5 | Scientific Reports

Fig. 5

From: Genomic insertion of ancestral uricase into human liver cells to determine metabolic consequences of pseudogenization

Fig. 5

Determination of intracellular uric acid-, triglyceride-, ATP-levels after spheroid starvation (0, 48 and 72 h), and Western blot analysis. a Intracellular uric acid levels of Huh spheroids. b Intracellular triglycerides levels of Huh spheroids. c Intracellular ATP levels of Huh spheroids. Values of uric acid, triglycerides and ATP are means ± SEM of three replicates. *p < 0.05, **p < 0.01, ***p < 0.005, and ****p < 0.0001. d Representative images of western blotting analysis of protein lysate of Huh spheroids. AMP kinase (AMPK), Acetyl-CoA carboxylase (ACC), phosphorylated protein (p).

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