Table 4 Differentially abundant major* bacterial phyla, genera, and KEGG pathways by ciliate fractions analyzed using MaAsLin2 (p  0.05).

From: Exploring the impact of forage-to-concentrate ratios on the ruminal bacteriome in vitro focusing on ciliate-associated bacteria

Ciliate fraction (CF)

Taxa

Dominant Group

Relative abundance (%)

Pooled SEM

TB

EAB

IAB

Phyla

Pseudomonadota

TB

3.78A

0.16C

1.30B

0.19

Verrucomicrobiota

1.61A

0.02C

0.97B

2.43

Fibrobacterota

0.77A

0B

0.38B

0.12

Bacteroidota

EAB

34.57B

51.99A

36.24B

0.12

Cyanobacteria

0.64C

1.85A

1.59B

0.27

Bacillota

IAB

0.76B

45.66B

53.77A

2.41

Spirochaetota

0.75B

0.27B

2.44A

0.12

Elusimicrobiota

0.74B

0.03B

2.25A

0.38

Planctomycetota

0.73A

0B

1.00A

0.18

Genera

Christensenellaceae R-7 group

TB

6.28A

4.33B

2.34B

0.47

Succiniclasticum

6.19A

0.02C

1.06B

0.23

Muribaculaceae

4.80A

2.60B

2.99B

0.53

Moryella

1.49A

0B

0B

0.04

Ruminococcaceae CAG-352

1.37A

0B

0B

0.05

Succinivibrio

1.11A

0B

0.11B

0.12

Pedosphaeraceae DEV114

0.85A

0C

0.48B

0.06

Prevotellaceae UCG-001

0.70A

0.02B

0.10B

0.08

Anaerovoracaceae Family XIII AD3011 group

0.60A

0.03B

0.06B

0.06

[Eubacterium] ruminantium group

0.58A

0B

0.06B

0.07

Veillonellaceae UCG-001

0.43A

0B

0B

0.06

Rikenellaceae RC9 gut group

EAB

13.55C

40.38A

21.49B

1.90

[Oscillospirales] UCG-010

1.65B

12.86A

0.96B

1.09

Prevotellaceae UCG-004

0.92B

2.67A

0.83B

0.19

Butyrivibrio

IAB

1.90B

0.04C

6.15A

0.76

Saccharofermentans

0.94B

0C

5.02A

0.60

Lachnoclostridium

0B

0B

1.54A

0.19

Lachnospiraceae XPB1014 group

0.63B

0C

3.71A

0.37

[Spirochaetota] MVP-15

0.53B

0.01C

1.18A

0.11

Lachnospiraceae AC2044 group

0.35B

0.01B

1.15A

0.20

KEGG pathways

KEGG pathways

Dominant group

Relative abundance (%)

Pooled SEM

Pathway

TB

EAB

IAB

ko05132

TB

3.93A

2.64B

0.10B

0.96

Salmonella infection

ko00630

1.22A

0.43B

0B

6.39

Glyoxylate and dicarboxylate metabolism

ko05143

EAB

1.71B

3.81A

1.14B

0.12

African trypanosomiasis

ko03010

2.97B

3.67A

3.28B

3.25

Ribosome

ko00780

2.80C

3.62A

3.11B

0.13

Biotin metabolism

ko04978

2.54B

2.94A

1.19B

0.22

Mineral absorption

ko01502

2.20B

2.83A

2.51B

2.58

Vancomycin resistance

ko03440

2.25B

2.74A

2.45B

2.43

Homologous recombination

ko00020

1.67B

2.46A

1.86B

2.40

Citrate cycle (TCA cycle)

ko04122

1.43B

1.77A

1.57B

1.60

Sulfur relay system

ko00450

1.54B

1.91A

1.76B

1.72

Selenocompound metabolism

ko03018

1.30B

1.62A

1.44B

1.45

RNA degradation

ko00190

0.79B

1.08A

0.90B

1.03

Oxidative phosphorylation

ko04141

0.10B

0.13A

0.11B

0.13

Protein processing in endoplasmic reticulum

ko01040

0.05B

0.08A

0.06B

0.32

Biosynthesis of unsaturated fatty acids

ko03450

0.04B

0.07A

0.02B

0.18

Non-homologous end-joining

ko00040

IAB

0.68B

0.16C

0.84A

2.83

Pentose and glucuronate interconversions

  1. *Major classified taxa occupying over 0.5% average relative abundance and present in 50% of the samples in at least one of the groups.
  2. A−C: significant differences (q  0.05) among groups.
  3. Diet, diet effect after 24 h of incubation (F:C ratio); CF, ciliate fraction effect after 24 h of incubation; TB, total bacteria; EAB, small entodinia-associated bacteria; IAB, Isotricha-associated bacteria; HC, high-concentrate group; CON, control group; HF, high-forage group; Pooled SEM, pooled standard error of the mean.