Fig. 2 | Scientific Reports

Fig. 2

From: HIF-1α regulates the proliferation and differentiation of mouse cranial base sphenoid-occipital synchondrosis chondrocytes via PI3K/Akt signaling

Fig. 2

Isolation, culture, and identification of SOSCs; (A) Schematic diagram of isolation and culture of mouse sphenooccipital synchondrosis cells; (B–D) First-generation SOSCs exhibit typical triangular or quadrilateral shapes; (E) Late-stage growth of SOSCs shows cobblestone-like arrangement; (F–H) Alcian blue staining, Safranin O staining, and toluidine blue staining demonstrate abundant proteoglycans in SOSCs; (I) Immunofluorescence staining of cells shows positive expression of type II collagen in the extracellular matrix of SOSCs; (J) CCK-8 assay detects the proliferation activity of SOSCs.

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