Table 3 Chlorophyll fluorescence parameters40.
Basic parameters derived from the extracted data | ||
|---|---|---|
F0 | Minimal fluorescence yield of the dark-adapted state | F0 = F50µs |
Fm | Maximal fluorescence yield of the dark-adapted state | Fm = Fp |
Fv | Variable fluorescence | Fv = Fm – F0 |
Fv/Fm | The maximal quantum yield of PSII photochemistry | Fv/Fm = 1– (F0/Fm) |
VJ | Relative variable fluorescence at time 2 ms (J-step) after the start of an actinic light pulse | VJ = (F2ms – F0)/(Fm – F0) |
VI | Relative variable fluorescence at time 30 ms (I-step) after the start of an actinic light pulse | VI = (F30ms – F0)/(Fm – F0) |
Area | The area above the OJIP curve; expresses the size of the reduced PQ pool | |
Sm | Normalized area related to the number of electron carriers per electron transport | Sm= (area/(Fm-Fo) |
Quantum yields | ||
|---|---|---|
Ψ0 | The probability that a trapped exaction moves an electron into the electron transport chain beyond | QA Ψ0 = (1 – VJ) |
φPo | The maximum quantum yield of primary PSII photochemistry | Ψo= (1-Vj) |
ΦDo | Quantum yield of energy dissipation | ΦDo = F0/FV |
φET20 | Quantum yield of electron transport from QA to QB in PSII | φET20 = ΦPo(1 – VJ) |
φRE10 | Quantum yield of reduction of end electron acceptors at the PSI acceptor side | φRE10 = ΦPo(1 – VI) |
PIabs | The performance index for the photochemical activity (basic formula on absorption basis) | PIabs = [1– (F0/Fm)]/(M0/VJ) × (Fm – F0)/F0 × (1 – VJ)/VJ |
PItot | The total performance index for the photochemical activity (including the flow beyond PSI) | PItot = PIabs(1 – VI)/(VI – VJ) |
TR0/RC | Trapped energy flux per RC | Mo(1/Vj) |
ET0/RC | Electron transport flux per RC | Mo(1/Vj)ψo |