Fig. 4
WD-fed Icam1tmBay mice exhibit ameliorated MASH progression. Wild type (WT) mice (shown in black) and Icam1tmBay mice (shown in purple) were fed for 24 weeks with chow diet (CD) or Western diet (WD). Depicted are the endpoint values of WT CD-fed (n = 4–13), WT WD-fed (n = 7–15), Icam1tmBay CD-fed (n = 6), and Icam1tmBay WD-fed (n = 6–8). (A) Quantification of serum aspartate aminotransferase (AST). (B) Quantification of serum alanine aminotransferase (ALT). (C) Quantification of alkaline phosphatase (ALP). (D) Representative images of H&E-stained liver sections of the indicated mice strains (original magnification X 20, scale bar = 50 μm). (E) Non-alcoholic fatty liver disease (NAFLD) activity score (NAS). (F) Representative images of Sirius red-stained liver sections of the indicated mice strains (original magnification X 20, scale bar = 50 μm). (G) Quantification of Sirius red-positive area expressed as a percentage of the tissue area. (H) mRNA levels of collagen 1alpha (col1a) in liver tissue homogenates. For quantification, values are expressed as fold increase over the mean values obtained for liver tissue from CD-fed WT mice. (I) Hepatic triglycerides in liver tissue homogenates. (J) Free fatty acids (FFA) in liver tissue homogenates. Statistical significance was calculated by the two-way ANOVA. Values are represented as mean ± SD (A-C, E, G, H). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Only differences between genotypes (WT and Icam1tmBay mice) within a diet (CD or WD) and between diets (CD and WD) within a genotype (WT and Icam1tmBay mice) were calculated.
