Fig. 5

Increased WD-induced hepatic neutrophil frequencies in Icam1^tmBay mice do not correlate with hepatic myeloperoxidase expression. Wild type (WT) mice (shown in black) and mice with dysfunctional ICAM-1 gene (Icam1^tmBay) mice (shown in purple) were fed for 24 weeks with chow diet (CD) or Western diet (WD). Depicted are the endpoint values of WT CD-fed (n = 4–6), WT WD-fed (n = 7–8), Icam1^tmBayCD-fed (n = 5–6), and Icam1^tmBayWD-fed (n = 7–8). (A) Comparative immune cell analysis of liver by flow cytometry. Representative FACS dot plots illustrating the gating strategy are shown in Supplementary Fig. S1. Depicted are the percentages of CD45⁺ cells of CD4⁺ T cells (CD45⁺CD3⁺CD8^-CD4⁺), CD8⁺ T cells (CD45⁺CD3⁺CD4^-CD8⁺), B cells (CD45⁺CD3^-CD19⁺), NK cells (CD45⁺NK1.1⁺), neutrophils (CD45⁺CD11b⁺Ly6G⁺), and macrophages (CD45⁺Ly6G^-CD11b⁺F4/80⁺). (B) mRNA levels of monocyte chemoattractant protein 1 (Mcp1) in liver tissue homogenates. For quantification, values are expressed as fold increase over the mean values obtained for liver tissue from CD-fed WT mice. (C) Representative images of myeloperoxidase (MPO) stained liver sections of the indicated mice strains (original magnification X 20, scale bar = 50 μm). (D) Quantification of MPO positive area expressed as a percentage of the tissue area. Statistical significance was calculated by two-way ANOVA. Values are represented as mean ± SD (B, C). * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001. Only differences between genotypes (WT and Icam1^tmBay mice) within a diet (CD or WD) and between diets (CD and WD) within a genotype (WT and Icam1^tmBay mice) were calculated.