Fig. 1 | Scientific Reports

Fig. 1

From: BDNF improves fracture healing through promoting osteoblasts proliferation and migration via trkb/akt regulated NUCKS1 expression

Fig. 1

Effects of BDNF on NUCKS1 expression in MC3T3-E1 cells and fracture-healing tissues (n = 3). (A) Western blots of NUCKS1 expression in MC3T3-E1 cells treated with 50 ng/mL BDNF for 12 h. (B) Statistical analysis of results in (A). (C) Statistical analysis of the results qRT-PCR analysis of NUCKS1 expression in MC3T3-E1 cells treated with 50 ng/mL BDNF for 12 h. (D) Western blots of NUCKS1 expression in the callus tissue after local administration of 100 ng/mL BDNF to the fracture site every 2 days for 2 weeks. E Statistical analysis of results in (D). Data in (B), (C), and (E) were analyzed using independent-samples t test; *p < 0.05, **p < 0.01, ***p < 0.001.

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