Fig. 5
From: Induction of proline-rich proteins in response to tannin treatment in Caenorhabditis elegans
C. elegans strain WWU202 expressing CLX-1::GFP after treatment with different stressors for 18 h. Representative GFP fluorescence images of the respective animals: (a) UC: Untreated control, (b) heat stress (30 °C), (c) osmotic stress (300 mM NaCl), (d) heavy metal stress (8 mM CdCl2), (e) oxidative stress (250 µM juglone), (f) untreated control in M9 buffer including 1% DMSO, (g) endoplasmic reticulum stress (5 µg/mL tunicamycin), (h-j) C. mucronatum extract at 0.02 mg/mL, 0.2 mg/mL and 2 mg/mL, (k) Geraniin (0.2 mM), (l) P. urinaria extract (0.2 mg/mL). Scale bar 50 μm. Experiments were performed in triplicate with at least ten animals per replicate.
