Fig. 6 | Scientific Reports

Fig. 6

From: Bisphenol A suppresses colon epithelial cell responses via G0/G1-phase arrest, MAPK and PI3K/AKT pathway modulation, and MMP-2/9 Inhibition by upregulating p21WAF1

Fig. 6

BPA suppresses MMP-2 and − 9 expression via inhibition of AP-1, Sp-1, and NF-κB binding in colon cells. (A) The activity and protein levels of MMP-2 and − 9 in HCT 116 and HCT-8 cells were assessed under gelatin zymography and immunoblotting. As the loading control, β-actin was employed in the immunoblot experiment. (B) The DNA-binding affinities of Sp-1, AP-1, and NF-κB in colon cells were determined by EMSA. All values from three triplicate experiment are expressed as mean ± standard deviation. *p < 0.05 compared with control. Original blots/gels are presented in Supplementary Fig. 11.

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