Fig. 8

p27KIP1 silencing with siRNA had no effect on the BPA-induced colon epithelial cell responses. Colon cells were transfected with either scramble siRNA or p27KIP1 siRNA (si-p27), and then treated with BPA for 24 h. Transfected cells were analyzed by MTT (A) and cell counting assays (B). Wound-healing migration (C) and invasion (D) assays were performed to estimate the metastatic ability using transfected cells. All values from triplicates data are designated as mean ± standard deviation. *p < 0.05 compared with control, #P < 0.05 compared with BPA treatment.