Fig. 2 | Scientific Reports

Fig. 2

From: Extracellular vesicles from different regions of the female reproductive tract promote spermatozoa motility and support capacitation

Fig. 2

EVs characterization. (a-f) Transmission electron microscopy (TEM) representative images of FF-EVs (a, b), CVF-EVs LH + 2 (c), CVF-EVs LH + 7 (d), dESCs-EVs (e) and eESCs-EVs (f). Scale bar: 500 nm. (g) Descriptive table reporting the average particle concentration, size and 90th percentile of the size (D90) obtained with NTA conducted on CVF-EVs LH + 2 (n = 3), CVF-EVs LH + 7 (n = 3), eESCs-EVs (n = 3), dESCs-EVs (n = 3) and FF-EVs (n = 2). (h) Western blot showing the presence of different canonical EV markers (Alix, TSG101, CD63, CD9) in representative samples of FF-EVs (n = 2), CVF-EVs LH + 2 and LH + 7 (n = 2) and of dESCs-EVs and eESCs-EVs (n = 1). Representative cell lysates from eESCs and dESCs (n = 1) were included as positive controls for CNX expression, used as EV negative marker.

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