Fig. 3
From: Estrogen receptor α regulates SVCT2 protein level in human breast cancer cells
ERα knockdown led to a reduction in the stability of SVCT2. (A, C) MCF7 and T47D Cells were transfected with scrambled siRNA or si-ERα and exposed to CHX (40 μM) for indicated times. Cell lysates were prepared for western blot analysis. (B, D) Cells were transfected with scrambled siRNA or si-ERα and exposed to MG132 (10 μM) or Leupeptin (10 μM) for 24 h, and whole cell lysates were immunoblotted as indicated. GAPDH was used as an internal control.
