Fig. 4 | Scientific Reports

Fig. 4

From: KLF5-regulated ZDHHC8 reduces chemosensitivity in high-grade serous ovarian cancer through β-catenin signaling

Fig. 4

KLF5 upregulates ZDHHC8 expression to impair cisplatin sensitivity. (a) Schematic illustrating the predicted KLF5 binding site within the ZDHHC8 promoter, identified using the JASPAR database. (b) Chromatin immunoprecipitation (ChIP) assay confirming KLF5 binding to the ZDHHC8 promoter region. (c) Luciferase reporter assay comparing ZDHHC8 promoter activity in wild-type and mutant constructs upon KLF5 overexpression. (d) Representative immunohistochemical images of KLF5 expression in clinical HGSOC samples (20× magnification). (e) QPCR and WB analysis verifying the efficiency of KLF5 knockdown (shKLF5) and overexpression (oeKLF5), and their effects on ZDHHC8 levels in HGSOC cells. (f) Flow cytometry analysis showing apoptosis levels in 10 µM CDDP-treated shKLF5 HGSOC cells. (g) γ-H2AX immunofluorescence staining showing DNA damage levels in 10 µM CDDP-treated shKLF5 cells.

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