Fig. 5
Immune landscape of acne with(n = 40) or without IR(n = 50). A Heatmap displays Z-score normalized expression profiles of nine cell populations in acne with IR and acne without IR groups, highlighting immune microenvironmental compositional differences. B Normalized enrichment scores (NES) of B-cells had significantly higher activity in the IR group (P = 7.55 E-3, Wilcoxon sign-rank test) and fibroblasts in the non-IR group (P = 6.85 E-3, Wilcoxon sign-rank test). C Five major signature differential molecules were enriched in B cells and three major signature differential molecules were enriched in fibroblasts. D Molecules positively correlated with B cell activity (Pearson’s R > 0, P < 0.05). E Pathways positively correlated with B cell activity in acne with IR group. F The molecular-specific correlations in representative pathways such as NF-κB signaling and Ras signaling (*P < 0.05, ** P < 0.01). G Molecules positively correlated with fibroblast activity (Pearson’s R > 0, P < 0.05). H Pathways positively correlated with fibroblast activity in the non-IR group. I The molecular-specific correlations in representative pathways such as the actin filament-based processes and platelet activation (* P < 0.05,** P < 0.01). J Summary schematic integrating key findings from panels A-I .
