Fig. 9

Western blot was used to determine the expression levels of HSPA5 in U118, U87, and U251 cells transfected with HSPA5-targeting siRNA (A). CCK8 assay was used to evaluate the cell growth capacity of U118, U87, and U251 cells transfected with HSPA5 siRNA over a period of 3 days (B). HSPA5 gene knockout inhibited colony formation in U118, U87, and U251 cells; the colony formation images were captured using a camera (C). Transwell migration and invasion assays demonstrated that HSPA5 knockout inhibited the migration and invasion of U118, U87, and U251 cells; the scale is 200 μm (D). Flow cytometry showed that the proportion of early apoptotic cells increased in U118, U87, and U251 cells transfected with HSPA5-targeting siRNA (E).