Table 1 Optimized gradient program for simultaneous separation of 12 WSVs.

From: Simultaneous quantification of 12 water-soluble vitamins in MDCK cell culture media by eco-friendly ion-pairing reagent-free HPLC-UV

Time (min)

Flow Rate (mL/min)

% MeOH (A)

% KH₂PO₄ (B)

0

0.9

5

95

10

0.9

5

95

20

0.9

45

55

30

0.9

5

95

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