Fig. 4
From: The P4HB/LGALS3BP/NF-kB axis promotes the progression of MIA to IAC in lung adenocarcinoma
LGALS3BP inhibited activation of the NF-kB pathway to promote the LUAD progression. (A) Detection of the effect of LGALS3BP on ROS levels in LUAD cells. (B) Immunostaining was used to detect the effect of LGALS3BP on the level of JC-1 in LUAD cells. Detection of ferroptosis markers (C) GSH and (D) MDA. (E) wound healing, (F) transwell, (G) CCK-8 and (H) clone formation were used to detect the effect of LGALS3BP on the proliferation, invasion, and migration ability of LUAD cells. Western blotting was used to detect the effect of LGALS3BP on the NF-kB pathway in (I) H1299 and (J) A549 cells (original blots/gels are presented in Supplementary Fig. 5). *, P < 0.05; **, P < 0.01.
