Fig. 2
TSG6 knockdown leads to reduced expression levels of tumor growth factors and multi-drug resistance genes in canine MGT cells. (A) Relative mRNA expression levels for of TGF-β, VEGF, MRP1, and P-gp in CIPp cells cultured as controls and transfected with si-ctrl or si-TSG6. (B) Relative mRNA expression levels for of TGF-β, VEGF, MRP1, and P-gp in CIPm cells cultured as controls and transfected with si-ctrl or si-TSG6. The results are presented as the mean ± SD and were from of triplicate samples from three independent experiments. TSG6, TNF-stimulated gene 6 protein; MGT, mammary gland tumor; TGF-β, Transforming growth factor β; VEGF, Vascular endothelial growth factor; MRP1, multi-drug resistance-related protein; P-gp, P-glycoprotein; ctrl, control. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, as determined by one-way ANOVA.
