Table 2 Primers and product size.
Gene | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|
RUNX2 | 5’-GACAGCCCCAACTTCCTGTG-3’ | 5’-CCGGAGCTCAGCAGAATAAT-3’ | 159 |
Osterix | 5’-TGCTTGAGGAGGAAGTTCAC-3’ | 5’-CTGCTTTGCCCAGAGTTGTT-3’ | 114 |
Osteocalcin | 5’-CTCACACTCCTCGCCCTATT-3’ | 5’-TCAGCCAACTCGTCACAGTC-3’ | 245 |
Collagen I | 5’-CCTGGATGCCATCAAAGTCT-3’ | 5’-AATCCATCGGTCATGCTCTC-3’ | 174 |
GAPDH | 5’-CAATGACCCCTTCATTGACC-3’ | 5’-TTGATTTTGGAGGGATCTCG-3’ | 159 |
