Fig. 5 | Scientific Reports

Fig. 5

From: N-acetylcysteine inhibits NETs, exhibits antibacterial and antibiofilm properties and enhances neutrophil function against Burkholderia pseudomallei

Fig. 5

CLSM images of biofilms when B. pseudomallei was co-cultivated with PMNs in the presence of NAC. Top row: CLSM images of the 48-h B. pseudomallei H777 biofilm of the control group in LB (LB). Second row: incubated in complete RPMI followed by LB (cR/LB). Third row: co-cultivated with PMNs at MOI 10 in cR/LB without NAC (+ PMNs, No NAC). Fourth row: incubated with PMNs and 15 mM NAC for 3 h (+ PMNs, 3 h NAC). Fifth row: incubated with PMNs and 15 mM NAC for 48 h (+ PMNs, 48 h NAC). The biofilms were stained with FITC-ConA (biofilm, green) and TOTO-3 (eDNA, red) before observation under CLSM (630×). B. and C. COMSTAT images analysis indicating the 48 h-biofilm biomass and eDNA quantity. Data from 12 images was used in each analysis, which display as mean ± SD. Asterisks indicate statistical significance (**p < 0.01, ***p < 0.001, ****p < 0.0001).

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