Fig. 8 | Scientific Reports

Fig. 8

From: N-acetylcysteine inhibits NETs, exhibits antibacterial and antibiofilm properties and enhances neutrophil function against Burkholderia pseudomallei

Fig. 8

Effect of NAC on B. pseudomallei survival in the presence of PMNs. The percentage survival of B. pseudomallei H777 cells when co-cultured with PMNs at 37 °C was assessed in the absence or presence of 1 unit/mL DNase (to degrade eDNA from NETs), 10 µg/mL cytochalasin D (to inhibit phagocytosis), both DNase and cytochalasin D, 15 mM NAC (to inhibit NETs and B. pseudomallei growth), along with untreated controls for 90 min. The percentage of surviving B. pseudomallei cells was calculated relative to the untreated controls. Results were obtained from three independent experiments, each done in triplicate (n = 9), expressed as mean ± SD. Asterisks indicated statistical significance (*p < 0.05, **p < 0.01, ****p < 0.0001).

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