Fig. 5
From: Extraction-free RT-RPA assay for detection of HPV16, HPV18, and HPV45 mRNA expression
Cervicovaginal samples tested at the point of care and with an RT-qPCR gold standard. Top: Cervicovaginal samples collected directly into 0.5% PVSA and tested onsite with the HPV16, HPV18, HPV45, and β-actin RT-RPA assays with (+ RT) and without (-RT) reverse transcriptase. N = 1. Bottom: Cervicovaginal samples collected into PreservCyt media with RNA extracted and tested on RT-qPCR for HPV16, HPV18, HPV45, and β-actin mRNA.
