Fig. 9

Function of MCM6 in lung adenocarcinoma cells. (A) Immunohistochemical analysis showed that the expression of MCM6 in lung adenocarcinoma tissues was higher than that in adjacent tissues. (B) The high expression of MCM6 was associated with poor prognosis of lung adenocarcinoma patients. (C) Interference efficiency verification of MCM6 knockdown by 3 different sequences of small interfering RNAs using RT-qPCR in A549 and H1299 cells. (D) Interference efficiency verification of MCM6 knockdown by 3 different sequences of small interfering RNAs using Western blotting in A549 and H1299 cells. (E,F) The in vitro cells proliferation function of MCM6 was measured by CCK-8 and colony formation experiment. (G) Flow cytometry was used to analyze cell cycle after MCM6 silenced in A549 cells. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. Control group (All experiments were independently repeated 3 times, and the data were expressed as mean +/- standard deviation). Note(C,D): The purpose of this part of the experiment is mainly to select the target with the highest knockdown efficiency for the next step of the experiment, and no comparisons among multiple groups are required.