Table 2 The primer sequences of genes for RT-qPCR
Gene | Primers sequences and directions (5’-3’) | Tm (℃) | Product size (bp) | GenBank accession no. | Primer efficiency (%) |
|---|---|---|---|---|---|
Il-1β | GAACTTAACCAGCTCTGAAATGATG CATGTCGCATCTGTAGCTCATTG | 58 | 103 | BC098597.1 | 100 |
Il-8 | TGTTTTCCTGGCATTTCTGACC TTTACAGTGTGGGCTTGGAGGG | 58 | 118 | ZDB-GENE 081104-317 | 98 |
Il-10 | TCACGTCATGAACGAGATCC CCTCTTGCATTTCACCATATC C | 57 | 149 | BC163031.1 | 100 |
Tgf-α | GGATGTTGAAGAAGGAGAG TTG TATGGAGCGTGAAGCAGAC | 59 | 185 | AY427649.1 | 100 |
Tgf-β | TGGCTCGATTTCTCAGTCGG CCCTCTGCTTCAGTCCATCTC | 58 | 211 | AY178450.1 | 99 |
Reference genes | |||||
Gapdh | CAGTCGCTGATGTGTCCGTTGTC GAGACGAACGCAGCGTCTCGTAG | 59 | 416 | NM_213094 | 100 |
β act | GTATTGCTGACCGTATGC CTGCCTCATCGTATTCCT | 58 | 139 | NM_131031.2 | 99 |
