Fig. 6
Effects of melatonin on plasma calcium levels in female goldfish. (A) Effects of different doses (0.1 μg, 1 μg, and 10 μg/g body weight) of exogenous melatonin on plasma calcium levels in female goldfish were evaluated at midnight (n = 9 or 10) in April 2010. The horizontal axis in the graph depicts the time (hours) after exogenous melatonin administration. Value at time point 0 was set as 100% for each result of the melatonin doses. *P < 0.05 indicates significant difference vs. control at 24 h after melatonin (10 μg/g body weight) injection. Close circle, control group; close triangle, 0.1 μg/g body weight melatonin-administered group; open triangle, 1 μg/g body weight melatonin-administered group; and open circle, 10 μg/g body weight melatonin-administered group. (B) Effects of exogenous melatonin (10 μg/g body weight) on plasma calcium levels in female goldfish were evaluated (each time point n = 8-12) in April 2009 (left graph) and February 2009 (right graph). The horizontal axis in each graph shows the time (hours) after exogenous melatonin administration. The value at time point 0 was set as 100% for each graph. *P < 0.05 and **P < 0.01 indicate significant differences vs. control at each time point. Close circle: control groups, open circle: melatonin-administrated groups.
