Fig. 7
Expression analysis of salt-responsive genes in A. thaliana under NaCl stress with or without Pseudomonas sp. A-2 treatment. RT-qPCR analysis was conducted on five salt stress–responsive genes: RD20 (a), RD29A (b), RD29B (c), and KIN1 (d) in 10-day-old A. thaliana seedlings co-cultivated with or without the A-2 strain (OD₆₀₀ = 0.1) under NaCl treatments (0, 100, and 150 mM) for 3, 6, and 12 h. Gene expression levels were calculated using the 2^–ΔCT method and normalized against actin2 as an endogenous control. Data represent the mean ± standard deviation of three biological replicates. Significant differences between control (C.) and Pseudomonas sp. A-2 treated plants (P. A-2) were evaluated using Student’s t-test : *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The results are presented as the mean ± standard deviation (n = 3).
