Fig. 1 | Scientific Reports

Fig. 1

From: Single cell spatial transcriptomics links Wnt signaling disruption to extracellular matrix development in a cleft palate model

Fig. 1

Whole transcriptome single cell spatial sequencing in cleft palate model reveals disruption in Wnt signaling and collagen organization.(a) Schematic representation of workflow for first whole transcriptome spatial sequencing in normal and abnormal palate development model. (b) Pseudo-bulk principial component analysis (PCA) of WT and Pax9−/− samples. (c-d) Functional enrichment analysis (FEA) showing an over representation test of the top 50 significant Biological Processes-Gene Ontology (BP-GO) terms of (c) E12.5 and (d) E13.5 wild type vs. KO embryonic palate tissues from Visium HD whole transcriptome spatial analysis. e-f) Spatial distribution maps (e) and violin plots (f) of differentially expressed genes, Sfrp2 (Wnt agonist) and Col1a1 (critical extracellular matrix structural component) both in the presence (WT) and absence (KO) of the functional protein, PAX9.

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