Fig. 6

Touchdown multiplex PCR amplification using newly designed primers to detect the Mat1-1 and Mat1-2 genes in Monilinia laxa, M. fructigena, and M. fructicola species on a 1.2% agarose gel. Isolates with mating type MAT1 exhibit a single band at 910 bp, while those with MAT2 show a single band at 1086 bp. (LAX: Monilinia laxa); (FRG: Monilinia fructigena); (FRC: Monilinia fructicola); (L: 100 bp ladder); (N: negative control).