Table 2 Four-factor, five level CCD and obtained responses for total experimental runs in the precipitation process.

Run order	Type	Decoded factor				Desirable function
Run order	Type	Conc. mg/ml	Ratio v/v	Addition Rate µl/min	Temp ° C	Desirable function
1	Center	50	5	600	32	53.455
2	Fact	40	3	800	28	60.572
3	Fact	60	3	800	36	62.653
4	Fact	40	3	400	36	60.618
5	Fact	60	7	800	28	77.300
6	Fact	40	7	800	36	62.432
7	Fact	40	7	400	28	61.240
8	Center	50	5	600	32	72.942
9	Fact	60	7	400	36	71.531
10	Fact	60	3	400	28	80.636
11	Fact	40	3	800	36	64.296
12	Center	50	5	600	32	68.041
13	Fact	60	7	800	36	66.689
14	Fact	60	3	800	28	81.241
15	Fact	40	7	400	36	82.624
16	Fact	60	7	400	28	83.694
17	Center	50	5	600	32	78.725
18	Fact	60	3	400	36	59.032
19	Fact	40	3	400	28	53.588
20	Fact	40	7	800	28	44.275
21	Axial	50	9	600	32	73.145
22	Axial	70	5	600	32	83.152
23	Axial	50	1	600	32	82.106
24	Axial	50	5	200	32	74.239
25	Axial	50	5	600	40	19.524
26	Center	50	5	600	32	75.552
27	Axial	50	5	600	24	57.617
28	Center	50	5	600	32	72.780
29	Axial	30	5	600	32	79.525
30	Axial	50	5	1000	32	76.715

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