Fig. 3 | Scientific Reports

Fig. 3

From: Refinement of the rat acetylcholine receptor-specific passive transfer myasthenia gravis model using subcutaneous injections: an update to the guidelines

Fig. 3

Decrement-inducing curare dose and muscle AChR content quantification. (A) The intraperitoneally infused curare dose during EMG. The amount of curare was determined by infusion speed and total time to achieve minimally 10% decrement. The curare dose corresponds to the number of functional AChR in the endplates. (B) Total AChR content in the TA was analysed by protein extraction followed by RIA. The amount of AChR was normalized to tissue weight and non-MG S.C. + I.P. values. The grey circled data point is the misinjected animal and has been excluded from statistical analysis. One way ANOVA with Bonferroni test was performed for statistical analysis. **P ≤ 0.01, ***P ≤ 0.001, **** P ≤ 0.0001 vs. non-MG S.C. + I.P.

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