Table 3 Primers and probes used for detection of the four BPV genotypes by ddPCR.
From: Accurate identification of bovine deltapapillomavirus in equine sarcoids by ddPCR
Forward 5′ 3′ | Reverse 5′ 3′ | Probe | region - size | |
|---|---|---|---|---|
BPV1 | ACTTCTGATCACTGCCATT | ATAGAAACCATAGATTTGGCA | TGAAGTGTTTCTGTTTGTGA-FAM | ORF E5 - 67 bp |
BPV2 | TACAGGTCTGCCCTTTTAAT | AACAGTAAACAAATCAAATCCA | AACAACAAAGCCAGTAACC-FAM | 3’UTR E5–77 bp |
BPV13 | CTGTGTGGATTTGATTTGTT | CAGGGGGAATACAAATTCT | TGAAGTGTTTCTGTTTGTGA-FAM | 3’UTR E5–98 bp |
BPV14 | CTTTGTTATTGTATATGAGTCTGT | ACTCTTGACGGTTTAAAAGTA | ATCTTGCCAGTGATCCTG-FAM | 3’UTR E5 − 98 bp |
