Fig. 1 | Scientific Reports

Fig. 1

From: Peptidoglycan-reshuffling proteins SCO0954, SCO1758, SCO4439, and SCO4440 modulate the formation of wall-deficient cells in Streptomyces coelicolor under hyperosmotic sucrose stress

Fig. 1The alternative text for this image may have been generated using AI.

Formation of wall-deficient cells and extracellular vesicles (EVs) under sucrose-induced hyperosmotic stress in the sco1760::Tn5, sco4439/40::Tn5062, and sco0954 knockout mutants, as well as in S. coelicolor overexpressing sco0954, grown on GYM medium supplemented with 0.64 M sucrose. Images correspond to confocal laser-scanning fluorescence microscopy of hyphae stained with SYTO9 and PI (DNA stains). Histograms represent EVs sizes (areas): grey bars correspond to the S. coelicolor wild-type strain (negative control, not producing EVs), used to establish the threshold area for EVs identification; blue bars represent EVs with an area above the 3.6 μm² threshold in mutants grown on GYM medium supplemented with 0.64 M sucrose. (a–d) Cultures on GYM medium supplemented with 0.64 M sucrose. (e–h) Cultures on GYM medium supplemented with 0.64 M sucrose. Representative images from at least three biological replicates are shown. Arrows indicate EVs and sucrose-driven hyperosmotic-stress-induced cells. Scale bars: 8 μm.

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