Fig. 3
Representative images of immunohistochemistry targeting storage material (GM1) within the retinas of 4- (A) and 7-month-old (C) wild type (WT) as well as 4- (B) and 7-month-old (D) Glb1 knockout (Glb1−/−) mice as well as BRN3A-positive neurons of the GCL in 7-month-old WT (E) and Glb1−/− (F) mice. Storage material is not detected in neurons of the ganglion cell layer in WT mice at both time points (A, C; thick arrows). Several neurons with brownish, granular, intracytoplasmic signals can be observed in 4- and 7-month old Glb1−/− mice (B,D; thin arrows). Some neurons without obvious intracytoplasmic signals can be observed, especially within 4-month-old Glb1−/− mice (B; thick arrow). In 7-month-old animals there is loss of visible neuronal nuclei (D; open arrowhead). Moreover, within the inner nuclear layer, scant (B; closed arrowheads) and more pronounced (D; closed arrowheads) accumulation of storage material can be detected. No positive signals were detected within the outer nuclear layer. A-F: scale bars: 20 μm. G,H: Graphs display box and whisker plots. Significant differences between groups were detected by Kruskal–Wallis test followed by Dunn’s–Bonferroni procedure; * p ≤ 0.05; n = 5. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer.
