Fig. 4 | Scientific Reports

Fig. 4

From: Novel insights into pathomechanisms of retinal neuronal degeneration and reactive gliosis in a murine model of GM1-gangliosidosis

Fig. 4

Immunohistochemistry targeting Iba1-positive cells within retinal layers. Wild type (WT) animals show low numbers of Iba1-positive cells in 4- (A) and 7-month-old mice (B). Increased numbers of Iba1-positive cells can be detected in 4- (C) and 7-(D) month-old Glb1 knockout (Glb1−/−) mice, especially in the ganglion cell layer (GCL) and inner plexiform layer (IPL). Scale bars: 50 μm. Quantification of total numbers of retinal Iba1 positive cells revealed significant differences between Glb1−/− and WT mice (E). Quantification of Iba1-positive cells according to different retinal layers shows a predominant increase in innermost retinal layers (GCL, IPL) compared to, for example, the outer plexiform layer (OPL) (F). Graphs display box and whisker plots. Significant differences between groups were detected by Kruskal-Wallis test followed by Dunn’s-Bonferroni procedure; * p ≤ 0.05; n = 5. INL, inner nuclear layer. Folds within the inner layers of retinal tissue have been digitally lightened.

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