Fig. 6 | Scientific Reports

Fig. 6

From: Novel insights into pathomechanisms of retinal neuronal degeneration and reactive gliosis in a murine model of GM1-gangliosidosis

Fig. 6

Immunofluorescence (IF) double staining of murine retinal tissue from a 7-month-old Glb1 knockout mouse. IF targeting GM1-1 shows an accumulation of GM1-positive lysosomal storage material (green; arrows) in ganglion cells (A,B). IF targeting glutamine synthetase (red, A) and GM1 (green) as well as glial fibrillary acidic protein (red, B) and GM1 (green) does not show colocalisation. This suggests that, in contrast to neurons of the RGC and INL (arrows), Müller cells are not affected by an excessive accumulation of storage material. Nuclei are counterstained with bisbenzimide (blue). Scale bars: 20 μm. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer.

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